Error-correcting Barcoded Primers for Pyrosequencing Hundreds of Samples in Multiplex

Nat Methods. 2008 Mar;5(3):235-7. doi: 10.1038/nmeth.1184. Epub 2008 Feb 10.

Abstract

We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of sample assignment errors, and thus characterized nearly as many 16S rRNA genes as have been sequenced to date by Sanger sequencing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primers / chemistry
  • Genetic Code
  • RNA, Bacterial / chemistry*
  • RNA, Ribosomal, 16S / chemistry*
  • Sequence Analysis, DNA / methods*

Substances

  • DNA Primers
  • RNA, Bacterial
  • RNA, Ribosomal, 16S