Minipreps of plasmid DNA

Curr Protoc Mol Biol. 2001 May:Chapter 1:Unit1.6. doi: 10.1002/0471142727.mb0106s15.

Abstract

Although there are a large number of protocols for the isolation of small quantities of plasmid DNA from bacterial cells (minipreps), this unit presents four procedures based on their speed and success: the alkaline lysis prep, a modification of the alkaline lysis prep that is performed in 1.5-ml tubes or 96-well microtiter dishes, the boiling method, and a lithium-based procedure. A support protocol provides information on storing plasmid DNA.

MeSH terms

  • Cloning, Molecular / methods*
  • DNA / isolation & purification*
  • Genetic Vectors / isolation & purification*
  • Molecular Biology / methods*
  • Plasmids / isolation & purification*

Substances

  • DNA