Abstract
This unit presents protocols outlining the methodology and techniques involved in the construction and application of HIV-1-based lentiviral vector systems. Also described are procedures that can be used to concentrate and purify high-titer recombinant lentiviral vector preparations, as well as protocols for transduction of adherent and suspension cells.
MeSH terms
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Cell Line
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Chemical Precipitation
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Chromatography, Ion Exchange / methods
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Defective Viruses / genetics
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Genes, gag
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Genes, tat
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Genetic Vectors / genetics*
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Genetic Vectors / isolation & purification
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HIV Reverse Transcriptase / analysis
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HIV-1 / genetics*
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HIV-1 / isolation & purification
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Humans
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Indicators and Reagents
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Kidney
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Polylysine
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Transduction, Genetic / methods
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Ultracentrifugation / methods
Substances
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Indicators and Reagents
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Polylysine
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reverse transcriptase, Human immunodeficiency virus 1
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HIV Reverse Transcriptase