Gene Silencing by RNAi in Mammalian Cells

Curr Protoc Mol Biol. 2003 May;Chapter 26:Unit 26.2. doi: 10.1002/0471142727.mb2602s62.

Abstract

This unit provides information how to use short interfering RNA (siRNA) for sequence specific gene silencing in mammalian cells. Several ways for siRNA generation and optimisation, as well as recommendations for cell transfection are presented.

MeSH terms

  • Animals
  • Cells, Cultured / metabolism
  • Electroporation / methods*
  • Genes, Reporter
  • Humans
  • Indicators and Reagents
  • Lipids
  • Liposomes
  • Mammals / genetics*
  • Nucleic Acid Hybridization / methods
  • RNA Interference*
  • RNA, Small Interfering / administration & dosage
  • RNA, Small Interfering / genetics*
  • Transfection / methods*

Substances

  • Indicators and Reagents
  • Lipids
  • Lipofectamine
  • Liposomes
  • RNA, Small Interfering