RNA interference in Caenorhabditis elegans

Curr Protoc Mol Biol. 2003 May:Chapter 26:Unit 26.3. doi: 10.1002/0471142727.mb2603s62.

Abstract

RNAi has become an essential tool in C. elegans research. Procedures for RNAi in C. elegans by microinjecting with dsRNA, feeding with bacteria expressing dsRNA and soaking in dsRNA solution are described in this unit.

MeSH terms

  • Administration, Oral
  • Animals
  • Base Sequence
  • Breeding
  • Caenorhabditis elegans / embryology
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / growth & development
  • Caenorhabditis elegans Proteins / genetics
  • Crosses, Genetic
  • Disorders of Sex Development
  • Gene Expression Regulation, Developmental
  • Homeodomain Proteins / genetics
  • Indicators and Reagents
  • Male
  • Microinjections
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • RNA Interference*
  • RNA, Double-Stranded / administration & dosage
  • RNA, Double-Stranded / genetics
  • RNA, Helminth / genetics*
  • RNA, Small Interfering / genetics*
  • Skin Absorption

Substances

  • Caenorhabditis elegans Proteins
  • Homeodomain Proteins
  • Indicators and Reagents
  • RNA, Double-Stranded
  • RNA, Helminth
  • RNA, Small Interfering
  • rde-1 protein, C elegans
  • unc-42 protein, C elegans