RNAi in transgenic plants

Curr Protoc Mol Biol. 2005 Nov;Chapter 26:Unit 26.6. doi: 10.1002/0471142727.mb2606s72.

Abstract

RNA silencing in plants is a rapid and facile approach for assignment of gene function and virus resistance. It allows selective RNA degradation through a mechanism that is given specificity by short interfering RNAs. The RNA silencer sequences are normally delivered as transgenes or a part of virus-vectors. This unit provides Basic Protocols for transgene and virus induced silencing. It also includes a Basic Protocol for protein overexpression using viral proteins that suppress silencing.

MeSH terms

  • Biomedical Research / methods*
  • Gene Silencing
  • Indicators and Reagents
  • Plants, Genetically Modified / genetics*
  • RNA, Double-Stranded / biosynthesis*
  • RNA, Plant / biosynthesis
  • RNA, Small Interfering / genetics*
  • Recombination, Genetic

Substances

  • Indicators and Reagents
  • RNA, Double-Stranded
  • RNA, Plant
  • RNA, Small Interfering