Recombinational cloning

Curr Protoc Mol Biol. 2006 May:Chapter 3:Unit 3.20. doi: 10.1002/0471142727.mb0320s74.

Abstract

The study of protein en masse, or functional proteomics, depends on the availability of full-length cDNAs in appropriate expression-ready plasmid vectors for protein expression and functional analysis. Recombinational cloning is a universal cloning technique based on site-specific recombination that is independent of the insert DNA sequence to be cloned, which differentiates this method from the classical restriction enzyme-based cloning methods. Recombinational cloning enables rapid and efficient parallel transfer of DNA inserts into multiple expression systems. This unit summarizes strategies for generating expression-ready clones using two of the most popular recombinational cloning technologies, now commercially available from Invitrogen (Gateway) and BD Clontech (Creator).

Publication types

  • Evaluation Study

MeSH terms

  • Cloning, Molecular / methods*
  • Models, Genetic
  • Polymerase Chain Reaction / methods
  • Proteomics / methods*
  • Recombination, Genetic*