Isolation of the ATP synthase subunit 6 and sequence of the mitochondrial ATP6 gene of the yeast Candida parapsilosis

Eur J Biochem. 1991 Apr 10;197(1):105-11. doi: 10.1111/j.1432-1033.1991.tb15887.x.


The mitochondrially translated product called subunit 6 was extracted from the yeast Candida parapsilosis mitochondria using an organic solvent mixture and purified by reverse-phase HPLC. The partial N-terminal sequence of subunit 6 reveals a post-translational cleavage site as in Saccharomyces cerevisiae. The structural mitochondrial gene ATP6 was isolated form a mitochondrial DNA library using the oligonucleotide probe procedure. The gene and the surrounding regions were cloned into M13tg130 and M13tg131 phage vectors. The insert contained an open reading frame 738-bp encoding a 246-amino-acid polypeptide. Mature subunit 6 contains 243 amino acid residues and the predicted molecular mass is 26,511 Da. The subunit shows 52% similarity with ATP synthase subunit 6 of the yeast S. cerevisiae. Comparison between protein and DNA sequences shows that the CUN codon family codes for a leucine in C. parapsilosis mitochondria.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Candida / enzymology*
  • Candida / genetics
  • Chromatography, High Pressure Liquid
  • Codon / genetics
  • DNA, Fungal / genetics*
  • DNA, Fungal / isolation & purification
  • DNA, Mitochondrial / genetics*
  • DNA, Mitochondrial / isolation & purification
  • Gene Library
  • Genes, Fungal*
  • Macromolecular Substances
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Protein Biosynthesis
  • Proton-Translocating ATPases / genetics*
  • Proton-Translocating ATPases / isolation & purification
  • Proton-Translocating ATPases / metabolism
  • Restriction Mapping
  • Saccharomyces cerevisiae / enzymology
  • Sequence Homology, Nucleic Acid


  • Codon
  • DNA, Fungal
  • DNA, Mitochondrial
  • Macromolecular Substances
  • Oligonucleotide Probes
  • Proton-Translocating ATPases

Associated data

  • GENBANK/X55653