Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression

Cell. 2008 Feb 8;132(3):487-98. doi: 10.1016/j.cell.2007.12.033.

Abstract

The cell-cycle transition from G1 to S phase has been difficult to visualize. We have harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop genetically encoded fluorescent probes for this purpose. These probes effectively label individual G1 phase nuclei red and those in S/G2/M phases green. We were able to generate cultured cells and transgenic mice constitutively expressing the cell-cycle probes, in which every cell nucleus exhibits either red or green fluorescence. We performed time-lapse imaging to explore the spatiotemporal patterns of cell-cycle dynamics during the epithelial-mesenchymal transition of cultured cells, the migration and differentiation of neural progenitors in brain slices, and the development of tumors across blood vessels in live mice. These mice and cell lines will serve as model systems permitting unprecedented spatial and temporal resolution to help us better understand how the cell cycle is coordinated with various biological events.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • COS Cells
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism
  • Cell Cycle*
  • Cell Line
  • Cells, Cultured
  • Chlorocebus aethiops
  • Cytological Techniques*
  • Fluorescence
  • Geminin
  • HeLa Cells
  • Humans
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Mice
  • Mice, Transgenic
  • Microscopy, Confocal
  • Molecular Sequence Data
  • Morphogenesis
  • Neoplasms / pathology
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Ubiquitination

Substances

  • CDT1 protein, human
  • Cell Cycle Proteins
  • GMNN protein, human
  • Geminin
  • Luminescent Proteins
  • Recombinant Fusion Proteins

Associated data

  • GENBANK/AB370332
  • GENBANK/AB370333