Cooperation and functional diversification of two closely related galactolipase genes for jasmonate biosynthesis

Dev Cell. 2008 Feb;14(2):183-92. doi: 10.1016/j.devcel.2007.11.010.


Jasmonic acid (JA) plays pivotal roles in diverse plant biological processes, including wound response. Chloroplast lipid hydrolysis is a critical step for JA biosynthesis, but the mechanism of this process remains elusive. We report here that DONGLE (DGL), a homolog of DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1), encodes a chloroplast-targeted lipase with strong galactolipase and weak phospholipase A(1) activity. DGL is expressed in the leaves and has a specific role in maintaining basal JA content under normal conditions, and this expression regulates vegetative growth and is required for a rapid JA burst after wounding. During wounding, DGL and DAD1 have partially redundant functions for JA production, but they show different induction kinetics, indicating temporally separated roles: DGL plays a role in the early phase of JA production, and DAD1 plays a role in the late phase of JA production. Whereas DGL and DAD1 are necessary and sufficient for JA production, phospholipase D appears to modulate wound response by stimulating DGL and DAD1 expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / enzymology
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis / ultrastructure
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism
  • Carboxylic Ester Hydrolases / genetics*
  • Chloroplasts / metabolism
  • Cyclopentanes / metabolism*
  • Gene Expression Regulation, Plant
  • Genes, Plant*
  • Genetic Variation*
  • Organ Specificity
  • Oxylipins / metabolism*
  • Phenotype
  • Phospholipase D / metabolism
  • Phospholipases A / metabolism
  • Phospholipases A1 / metabolism
  • Plant Leaves / growth & development
  • Plant Leaves / metabolism
  • Plant Stems / growth & development
  • Plant Stems / metabolism
  • Seedlings / ultrastructure
  • Transcriptional Activation / genetics


  • Arabidopsis Proteins
  • Cyclopentanes
  • Oxylipins
  • jasmonic acid
  • Carboxylic Ester Hydrolases
  • galactolipase
  • DAD1 protein, Arabidopsis
  • Phospholipases A
  • Phospholipases A1
  • Phospholipase D