Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow

J Exp Med. 1991 May 1;173(5):1213-25. doi: 10.1084/jem.173.5.1213.


We have resolved B220+ IgM- B-lineage cells in mouse bone marrow into four fractions based on differential cell surface expression of determinants recognized by S7 (leukosialin, CD43), BP-1, and 30F1 (heat stable antigen). Functional differences among these fractions can be correlated with Ig gene rearrangement status. The largest fraction, lacking S7, consists of pre-B cells whereas the others, expressing S7, include B lineage cells before pre-B. These S7+ fractions, provisionally termed Fr. A, Fr. B, and Fr. C, can differentiate in a stromal layer culture system. Phenotypic alteration during such culture suggests an ordering of these stages from Fr. A to Fr. B to Fr. C and thence to S7- pre-B cells. Using polymerase chain reaction amplification with pairs of oligonucleotide primers for regions 5' of JH1, DFL16.1, and Jk1, we find that the Ig genes of Fr. A are in germline configuration, whereas Fr. B and C are pro-B cell stages with increasing D-J rearrangement, but no V-D-J. Finally, functional analysis demonstrates that the proliferative response to IL-7, an early B lineage growth factor, is restricted to S7+ stages and, furthermore, that an additional, cell contact-mediated signal is essential for survival of Fr. A.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, CD*
  • Antigens, Surface / genetics
  • Antigens, Surface / immunology
  • Antigens, Surface / metabolism
  • B-Lymphocytes / cytology*
  • B-Lymphocytes / immunology
  • B-Lymphocytes / metabolism
  • Base Sequence
  • Biotin / metabolism
  • Bone Marrow / immunology
  • Bone Marrow / metabolism
  • Bone Marrow Cells*
  • Cell Differentiation / physiology
  • Cell Membrane / metabolism
  • Cell Membrane / ultrastructure
  • Cells, Cultured
  • DNA / analysis
  • DNA / genetics
  • Female
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain / genetics
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain / immunology
  • Gene Rearrangement, B-Lymphocyte, Light Chain / genetics
  • Gene Rearrangement, B-Lymphocyte, Light Chain / immunology
  • Hematopoietic Stem Cells / cytology*
  • Hematopoietic Stem Cells / immunology
  • Hematopoietic Stem Cells / metabolism
  • Immunoglobulin Heavy Chains / genetics
  • Immunoglobulin Heavy Chains / immunology
  • Immunoglobulin Heavy Chains / metabolism
  • Leukosialin
  • Mice
  • Molecular Sequence Data
  • Peptide Fragments / genetics
  • Peptide Fragments / immunology
  • Peptide Fragments / metabolism
  • Phenotype
  • Phycoerythrin / metabolism
  • Polymerase Chain Reaction
  • Sialoglycoproteins / metabolism


  • Antigens, CD
  • Antigens, Surface
  • Immunoglobulin Heavy Chains
  • Leukosialin
  • Peptide Fragments
  • Sialoglycoproteins
  • Spn protein, mouse
  • Phycoerythrin
  • Biotin
  • DNA