Distribution and characterization of immunoreactive porcine C-type natriuretic peptide

Biochem Biophys Res Commun. 1991 Mar 29;175(3):759-67. doi: 10.1016/0006-291x(91)91631-l.

Abstract

C-type natriuretic peptide (CNP) is a new member of the natriuretic peptide family recently identified in porcine brain (1). We raised an antiserum against porcine CNP and set up a radioimmunoassay (RIA) for CNP. Using this RIA system, distribution of immunoreactive (ir-) CNP in porcine tissue was measured and compared with that of ir-atrial natriuretic peptide (ANP) and ir-brain natriuretic peptide (BNP). Tissue concentration of ir-CNP in brain was the highest of the three natriuretic peptides at about 0.79 pmol/g wet wt. CNP was present in medulla-pons in high concentration, with a significant concentration detected in cerebellum. In contrast, ir-CNP was not detected in peripheral tissue, including heart, in a significant concentration. These data demonstrated sharp contrasts in the distribution of the three natriuretic peptides, suggesting that CNP is a natriuretic peptide functioning in the central nervous system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Atrial Natriuretic Factor / analysis
  • Brain Chemistry*
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Immune Sera
  • Immunoenzyme Techniques
  • Natriuretic Peptide, C-Type
  • Nerve Tissue Proteins / analysis*
  • Nerve Tissue Proteins / immunology
  • Organ Specificity
  • Radioimmunoassay
  • Spinal Cord / chemistry*
  • Swine

Substances

  • Immune Sera
  • Nerve Tissue Proteins
  • Natriuretic Peptide, C-Type
  • Atrial Natriuretic Factor