Probiotics and blueberry attenuate the severity of dextran sulfate sodium (DSS)-induced colitis

Dig Dis Sci. 2008 Sep;53(9):2464-73. doi: 10.1007/s10620-007-0174-x. Epub 2008 Feb 15.

Abstract

We studied the anti-inflammatory properties of probiotic strains and blueberry in a colitis model. The disease activity index (DAI) was significantly lower on days 9 and 10 in all groups compared to the colitis control. Myeloperoxidase (MPO) and bacterial translocation to the liver and to the mesenteric lymph nodes (MLN) decreased significantly in all groups compared to colitis control. Cecal Enterobacteriaceae count decreased significantly in blueberry with and without probiotics compared to the other groups. Lactobacillus plantarum reisolated from the cecal content in the presence of blueberry, contrary to Lactobacillus fermentum. Colonic MDA decreased significantly in all groups, except the L. fermentum group, compared to the colitis control. The cecal concentration of acetic, propionic, and butyricbutyric acid was significantly higher in the L. plantarum group, while the L. fermentum group yielded the highest concentration of lactic acid compared with all other groups. Lactobacillus plantarum DSM 15313, Lactobacillus fermentum 35D, and blueberry alone and in combination improve the DAI, reduce bacterial translocation, and reduce inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacterial Translocation
  • Blueberry Plants*
  • Chemokine CCL2 / metabolism
  • Chemokine CXCL1 / metabolism
  • Colitis / chemically induced
  • Colitis / diet therapy*
  • Colitis / metabolism
  • Colitis / microbiology*
  • Colon / drug effects
  • Colon / metabolism
  • Colon / microbiology
  • Dextran Sulfate
  • Disease Models, Animal
  • Enterobacteriaceae / growth & development
  • Enterobacteriaceae / isolation & purification
  • Fatty Acids, Volatile / metabolism
  • Lactobacillus plantarum / isolation & purification
  • Lipid Peroxidation / physiology
  • Liver / microbiology
  • Lymph Nodes / microbiology
  • Peroxidase / metabolism
  • Probiotics / therapeutic use*
  • Rats
  • Rats, Sprague-Dawley
  • Severity of Illness Index*

Substances

  • Ccl2 protein, rat
  • Chemokine CCL2
  • Chemokine CXCL1
  • Cxcl1 protein, rat
  • Fatty Acids, Volatile
  • Dextran Sulfate
  • Peroxidase