[Effects of millimeter wave on gene expression in human keratinocytes]

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2008 Jan;37(1):23-8. doi: 10.3785/j.issn.1008-9292.2008.01.005.
[Article in Chinese]


Objective: To explore the effect of millimeter wave exposure at low power density on gene expression in human keratinocytes (HaCaT).

Methods: HaCaT keratinocytes were exposed to 30.16 GHz millimeter wave with power densities of 1.0 or 3.5 mW/cm2 for 30 min per day. Gene expression profiles were obtained using the Affymetrix human genome U95A GeneChip. Reverse-transcription polymerase chain reaction (RT-PCR) was performed to confirm the differential expression of genes obtained from Genechip analysis.

Result: PAR-2 and ERGIC-53 genes in HaCaT cells were up-regulated by 3.5 mW/cm2 millimeter wave exposure for 4 times. ERGIC-53 gene was also up-regulated by 1.0 mW/cm2 millimeter wave exposure for 4 times. However, no significant change for PAR-2 expression was found after the same exposure.

Conclusion: Millimeter wave exposure could affect gene expression in human keratinocytes, which might be related to the intensity and the times of exposure.

Publication types

  • English Abstract

MeSH terms

  • Cells, Cultured
  • Dose-Response Relationship, Radiation
  • Electromagnetic Fields
  • Gene Expression / radiation effects*
  • Humans
  • Keratinocytes / metabolism
  • Keratinocytes / radiation effects*
  • Mannose-Binding Lectins / genetics
  • Mannose-Binding Lectins / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Microwaves*
  • Radiation
  • Receptor, PAR-2 / genetics
  • Receptor, PAR-2 / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Skin / cytology


  • LMAN1 protein, human
  • Mannose-Binding Lectins
  • Membrane Proteins
  • Receptor, PAR-2