Identification of phoslactomycin E as a metabolite inducing hyphal morphological abnormalities in Aspergillus fumigatus IFO 5840

J Antibiot (Tokyo). 2007 Dec;60(12):762-5. doi: 10.1038/ja.2007.101.

Abstract

In our survey for antifungal compounds, a fermentation broth of Streptomyces sp. HA81-2 was found to inhibit the in vitro growth of Aspergillus fumigatus IFO 5840 accompanied by hyphal morphological abnormalities. One of the isolated antibiotics was identified as phoslactomycin E based on LC-MS and NMR spectral data. In a preliminary assay using the membrane fractions of A. fumigatus, phoslactomycin E was found to inhibit the activity of 1,3-beta glucan synthase.

Publication types

  • Letter

MeSH terms

  • Antifungal Agents / chemistry
  • Antifungal Agents / isolation & purification
  • Antifungal Agents / pharmacology*
  • Aspergillus fumigatus / cytology*
  • Aspergillus fumigatus / drug effects*
  • Aspergillus fumigatus / growth & development
  • Chromatography, Liquid
  • Culture Media / chemistry
  • Fungal Proteins / antagonists & inhibitors
  • Glucosyltransferases / antagonists & inhibitors
  • Hyphae / cytology*
  • Hyphae / drug effects*
  • Lactones / chemistry
  • Lactones / isolation & purification
  • Lactones / pharmacology
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Molecular Structure
  • Organophosphorus Compounds / chemistry
  • Organophosphorus Compounds / isolation & purification
  • Organophosphorus Compounds / pharmacology
  • Streptomyces / metabolism

Substances

  • Antifungal Agents
  • Culture Media
  • Fungal Proteins
  • Lactones
  • Organophosphorus Compounds
  • phoslactomycin E
  • Glucosyltransferases
  • 1,3-beta-glucan synthase