Abstract
In our survey for antifungal compounds, a fermentation broth of Streptomyces sp. HA81-2 was found to inhibit the in vitro growth of Aspergillus fumigatus IFO 5840 accompanied by hyphal morphological abnormalities. One of the isolated antibiotics was identified as phoslactomycin E based on LC-MS and NMR spectral data. In a preliminary assay using the membrane fractions of A. fumigatus, phoslactomycin E was found to inhibit the activity of 1,3-beta glucan synthase.
MeSH terms
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Antifungal Agents / chemistry
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Antifungal Agents / isolation & purification
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Antifungal Agents / pharmacology*
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Aspergillus fumigatus / cytology*
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Aspergillus fumigatus / drug effects*
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Aspergillus fumigatus / growth & development
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Chromatography, Liquid
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Culture Media / chemistry
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Fungal Proteins / antagonists & inhibitors
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Glucosyltransferases / antagonists & inhibitors
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Hyphae / cytology*
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Hyphae / drug effects*
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Lactones / chemistry
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Lactones / isolation & purification
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Lactones / pharmacology
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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Molecular Structure
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Organophosphorus Compounds / chemistry
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Organophosphorus Compounds / isolation & purification
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Organophosphorus Compounds / pharmacology
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Streptomyces / metabolism
Substances
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Antifungal Agents
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Culture Media
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Fungal Proteins
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Lactones
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Organophosphorus Compounds
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phoslactomycin E
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Glucosyltransferases
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1,3-beta-glucan synthase