Persistent human immunodeficiency virus-1 antigenaemia affects the expression of interleukin-7Ralpha on central and effector memory CD4+ and CD8+ T cell subsets

Clin Exp Immunol. 2008 Apr;152(1):72-80. doi: 10.1111/j.1365-2249.2008.03610.x. Epub 2008 Feb 14.


Interleukin (IL)-7 and its receptor (IL-7Ralpha) play important roles in regulating lymphopoiesis. Previous studies have reported that human immunodeficiency virus-1 (HIV-1) viraemia affects the expression of IL-7Ralpha, but its effects on CD4+ and CD8+ T cell memory subsets have not been studied. Using eight-colour flow cytometry, we compared the immunophenotypic patterns of CD4+ and CD8+ T cell subsets expressing IL-7Ralpha and activation markers, as well as circulating IL-7 levels, in three well-defined groups of HIV-1-infected subjects: successfully treated, viraemic and long-term non-progressor (LTNP). Compared with successfully treated and LTNP subjects, viraemic patients had reduced expression of IL-7Ralpha on both CD4+ and CD8+ T cells, particularly on central and effector memory T cell compartments, and substantially elevated expression of activation markers on CD8+ T cell subsets. Circulating IL-7 levels were correlated negatively with the number of CD4+ and CD8+ T cell subsets expressing IL-7Ralpha; these associations were stronger with CD4+ T cell subsets and mainly with central and effector memory cells. The expression of activation markers on CD4+ and CD8+ cell T subsets was not related to circulating IL-7 levels. A strong negative correlation was observed between central memory CD4+ or CD8+ T cells expressing IL-7Ralpha and those expressing activation markers, independently of IL-7 levels. Collectively, these results provide further insight on the role of unsuppressed viral load in disrupting the IL-7/IL-7Ralpha system and contributing to HIV-1 disease progression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Viral / blood*
  • CD4 Lymphocyte Count
  • CD8-Positive T-Lymphocytes / immunology
  • Disease Progression
  • Female
  • Follow-Up Studies
  • HIV Infections / immunology*
  • HIV Infections / virology
  • HIV Long-Term Survivors
  • HIV-1 / immunology*
  • HIV-1 / isolation & purification
  • Humans
  • Immunophenotyping
  • Interleukin-7 / blood
  • Interleukin-7 Receptor alpha Subunit / blood*
  • Lymphocyte Activation / immunology
  • Male
  • T-Lymphocyte Subsets / immunology*
  • Viral Load


  • Antigens, Viral
  • Interleukin-7
  • Interleukin-7 Receptor alpha Subunit