Expression of cytokines and cytokine receptors in human Schwann cells

Neuroreport. 2008 Jan 8;19(1):31-5. doi: 10.1097/WNR.0b013e3282f27e60.

Abstract

The expression of cytokines and cytokine receptors was investigated in enriched populations of human fetal Schwann cells by reverse transcribed-PCR and enzyme-linked immunosorbent assay. Human fetal Schwann cells constitutively expressed mRNA of IL-1beta, IL-6, IL-8, IL-11, IL-12, IL-15 and TGF-beta, and also cytokine receptors for IL-1, IL-4, IL-6, IL-8, IL-13, tissue necrosis factor and gp130. The expression of IL-1beta, IL-6 and IL-15 was upregulated following treatment with IL-1beta or TGF-beta. The protein levels of IL-6 were increased with IL-1beta treatment, but were decreased with IFN-gamma treatment. Human Schwann cells may respond to cytokine signals in the nerve injury sites and modify the pathological conditions by secreting cytokines. The secreted cytokines may play a role in leukocyte recruitment and exacerbation of axonal injury process.

MeSH terms

  • Cells, Cultured
  • Cytokines / genetics
  • Cytokines / metabolism*
  • Cytokines / pharmacology
  • Enzyme-Linked Immunosorbent Assay / methods
  • Ganglia, Spinal / cytology
  • Humans
  • RNA, Messenger / metabolism
  • Receptors, Cytokine / genetics
  • Receptors, Cytokine / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction / methods
  • Schwann Cells / metabolism*
  • Up-Regulation / drug effects
  • Up-Regulation / physiology

Substances

  • Cytokines
  • RNA, Messenger
  • Receptors, Cytokine