SGV1 encodes a CDC28/cdc2-related kinase required for a G alpha subunit-mediated adaptive response to pheromone in S. cerevisiae

Cell. 1991 May 31;65(5):785-95. doi: 10.1016/0092-8674(91)90386-d.


The GPA1 gene of S. cerevisiae encodes a G alpha subunit that plays a positive role in the transduction of signals stimulating recovery from pheromone-induced cell cycle arrest. The GPA1Val50 mutation, in which Gly-50 is replaced by valine, causes hyperadaptation to pheromone. However, GPA1Val50 cells do not recover from division arrest in the absence of both CLN1 and CLN3, which encode G1 cyclins, indicating that the recovery-promoting activity of GPA1Val50 requires the function of G1 cyclins. An sgv1 mutation suppresses the hyperadaptive response caused by GPA1Val50 and also confers cold- and temperature-sensitive growth. The SGV1 gene encodes an apparent protein kinase homologous to CDC28/cdc2 kinase: SGV1 is 42% identical to CDC28. The activated mutation, CLN3-2, partially suppresses the growth defect of sgv1, suggesting that the SGV1 and CLN3 proteins may act in the same growth control pathway.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • CDC2 Protein Kinase / genetics*
  • CDC2 Protein Kinase / metabolism
  • CDC2-CDC28 Kinases
  • Cloning, Molecular
  • Cyclins / genetics*
  • Cyclins / physiology
  • GTP-Binding Proteins / physiology*
  • Genes, Fungal*
  • Genotype
  • Mating Factor
  • Molecular Sequence Data
  • Mutagenesis
  • Peptides / physiology*
  • Phenotype
  • Pheromones / physiology*
  • Protein Kinases / genetics*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / physiology
  • Sequence Homology, Nucleic Acid
  • Signal Transduction


  • Cyclins
  • Peptides
  • Pheromones
  • Mating Factor
  • Protein Kinases
  • CDC2 Protein Kinase
  • CDC2-CDC28 Kinases
  • GTP-Binding Proteins

Associated data

  • GENBANK/D90317