The T/E1A-binding domain of the retinoblastoma product can interact selectively with a sequence-specific DNA-binding protein

Cell. 1991 Jun 14;65(6):1073-82. doi: 10.1016/0092-8674(91)90559-h.


A DNA-binding site selection and enrichment procedure revealed a sequence-specific DNA-binding activity selectively associated with glutathione S-transferase-retinoblastoma protein chimeras (GST-RB) that had been incubated with a human cell extract. Appropriate mutant forms of GST-RB, incubated in equivalent extracts, did not associate with this specific DNA-binding activity, and a peptide replica of the HPV E7 RB-binding segment selectively inhibited the association of GST-RB with the sequence-specific DNA-binding protein(s). Sequence analysis of oligonucleotides with high affinity for GST-RB complexes, as well as the results of competition binding studies, strongly suggest that RB can associate specifically with the transcription factor E2F or with a protein having closely related DNA-binding properties.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenovirus Early Proteins
  • Base Sequence
  • Binding Sites
  • Carrier Proteins*
  • Cell Cycle Proteins*
  • DNA-Binding Proteins / metabolism*
  • E2F Transcription Factors
  • Glutathione Transferase / genetics
  • Macromolecular Substances
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / metabolism
  • Oncogene Proteins, Viral / metabolism*
  • Protein Binding
  • Recombinant Fusion Proteins
  • Regulatory Sequences, Nucleic Acid
  • Retinoblastoma Protein / metabolism*
  • Retinoblastoma-Binding Protein 1
  • Transcription Factor DP1
  • Transcription Factors / metabolism*
  • Transcription, Genetic


  • Adenovirus Early Proteins
  • Carrier Proteins
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • E2F Transcription Factors
  • Macromolecular Substances
  • Oligodeoxyribonucleotides
  • Oncogene Proteins, Viral
  • Recombinant Fusion Proteins
  • Retinoblastoma Protein
  • Retinoblastoma-Binding Protein 1
  • Transcription Factor DP1
  • Transcription Factors
  • Glutathione Transferase