Multiple forms of dynamin are encoded by shibire, a Drosophila gene involved in endocytosis

Nature. 1991 Jun 13;351(6327):583-6. doi: 10.1038/351583a0.


Dynamin was discovered in bovine brain tissue as a nucleotide-sensitive microtubule-binding protein of relative molecular mass 100,000. It was found to cross-link microtubules into highly ordered bundles, and appeared to have a role in intermicrotubule sliding in vitro. Cloning and sequencing of rat brain dynamin complementary DNA identified an N-terminal region of about 300 amino acids which contained the three consensus elements characteristic of GTP-binding proteins. Extensive homology was found between this domain and the mammalian Mx proteins which are involved in interferon-induced viral resistance, and with the product of the VPS1 locus in Saccharomyces cerevisiae, which has been implicated both in membrane protein sorting, and in meiotic spindle pole separation. Dynamin-containing microtubule bundles were not observed in an immunofluorescence study of cultured mammalian cells, but a role for a GTP-requiring protein in intermicrotubule sliding during mitosis in plants has been reported. We report here that Drosophila melanogaster contains multiple tissue-specific and developmentally-regulated forms of dynamin, which are products of the shibire locus previously implicated in endocytic protein sorting.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Blotting, Western
  • Ca(2+) Mg(2+)-ATPase / genetics*
  • Ca(2+) Mg(2+)-ATPase / immunology
  • Cloning, Molecular
  • Cross Reactions
  • DNA / genetics
  • Drosophila Proteins*
  • Drosophila melanogaster / genetics*
  • Dynamins
  • Endocytosis*
  • Genes
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Rats


  • Drosophila Proteins
  • DNA
  • Ca(2+) Mg(2+)-ATPase
  • Dynamins
  • shi protein, Drosophila