Fullerenol C60(OH)24 effects on antioxidative enzymes activity in irradiated human erythroleukemia cell line

J Radiat Res. 2008 May;49(3):321-7. doi: 10.1269/jrr.07092. Epub 2008 Feb 16.


Radiotherapy-induced toxicity is a major dose-limiting factor in anti-cancer treatment. Ionizing radiation leads to the formation of reactive oxygen and nitrogen species (ROS/RNS) that are associated with radiation-induced cell death. Investigations of biological effects of fullerenol have provided evidence for its ROS/RNS scavenger properties in vitro and radioprotective efficiency in vivo. Therefore we were interested to evaluate its radioprotective properties in vitro in the human erythroleukemia cell line. Pre-treatment of irradiated cells by fullerenol exerted statistically significant effects on cell numbers and the response of antioxidative enzymes to X-ray irradiation-induced oxidative stress in cells. Our study provides evidence that the pre-treatment with fullerenol enhanced the enzymatic activity of superoxide dismutase and glutathione peroxidase in irradiated K562 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Free Radical Scavengers / pharmacology*
  • Fullerenes / pharmacology*
  • Glutathione Peroxidase / metabolism
  • Humans
  • K562 Cells
  • Leukemia, Erythroblastic, Acute / enzymology*
  • Radiation-Protective Agents / pharmacology*
  • Superoxide Dismutase / metabolism


  • Free Radical Scavengers
  • Fullerenes
  • Radiation-Protective Agents
  • fullerenol
  • Glutathione Peroxidase
  • Superoxide Dismutase