Tissue sample collection for proteomics analysis

Methods Mol Biol. 2008;428:43-53. doi: 10.1007/978-1-59745-117-8_3.

Abstract

Successful collection of tissue samples for molecular analysis requires critical considerations. We describe here our procedure for tissue specimen collection for proteomic purposes with emphasis on the most important steps, including timing issues and the procedures for immediate freezing, storage, and microdissection of the cells of interest or "tissue targets" and the lysates for protein isolation for SELDI, MALDI, and 2DGE applications. The pathologist is at the cornerstone of this process and is an invaluable collaborator. In most institutions, pathologists are responsible for "tissue custody," and they closely supervise the tissue bank. In addition, they are optimally trained in histopathology in order to they assist investigators to correlate tissue morphology with molecular findings. In recent years, the advent of the laser capture microscope, a tool ideally designed for pathologists, has tremendously facilitated the efficiency of collecting tissue targets for molecular analysis.

MeSH terms

  • Electrophoresis, Gel, Two-Dimensional
  • Fluorescent Antibody Technique
  • Frozen Sections
  • Histological Techniques
  • Humans
  • Keratins / metabolism
  • Male
  • Microscopy, Confocal
  • Pathology, Clinical / methods
  • Prostate / anatomy & histology
  • Prostate / metabolism
  • Proteome / isolation & purification
  • Proteomics / methods*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Tissue Banks

Substances

  • Proteome
  • Keratins