Electrospun nanofiber scaffolds for rapid and rich capture of bone marrow-derived hematopoietic stem cells

Biomaterials. 2008 May;29(13):2096-103. doi: 10.1016/j.biomaterials.2008.01.024.

Abstract

Interactions between bone marrow-derived hematopoietic stem cells (BM-HSCs) and their local microenvironment are an integral part of signaling control of BM-HSCs migration, proliferation and differentiation. We hypothesized that both substrate topographical and biochemical cues promote BM-HSCs adhesive behaviors, which are crucial for BM-HSCs' homing, self-renewal and lineage commitment within their microenvironment. We employed electrospinning technique to fabricate nanofiber scaffolds (NFS) with poly(dl-lactide-co-glycolide) blended with collagen I. NFS was further coated with E-selectin, a critical adhesive biomolecule. Capture efficiency study showed that blended NFS, after coated with E-selectin, significantly increased cell capture percentage from 23.40% to 67.41% within 30 min and from 29.44% to 70.19% within 60 min of incubation at room temperature. This study highlights the potential of using a biomimetic scaffold to design a site-specific niche-like unit for facilitating proliferation or differentiation functions of BM-HSCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Marrow Cells / cytology*
  • Cell Differentiation*
  • Cells, Cultured
  • Collagen / chemistry
  • Collagen / ultrastructure
  • Electrons*
  • Hematopoietic Stem Cells / cytology*
  • Humans
  • Lactic Acid / chemistry
  • Microscopy, Electron, Scanning
  • Nanostructures / chemistry*
  • Nanostructures / ultrastructure
  • Polyglycolic Acid / chemistry
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Spectroscopy, Fourier Transform Infrared
  • Time Factors
  • Water / chemistry

Substances

  • Water
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Lactic Acid
  • Collagen