Effect of Grape (Vitis Vinifera L.) Leaf Extract on Alcohol Induced Oxidative Stress in Rats

Food Chem Toxicol. 2008 May;46(5):1627-34. doi: 10.1016/j.fct.2008.01.003. Epub 2008 Jan 8.

Abstract

Alcoholic liver disease is a major medical complication of drinking alcohol. Oxidative stress plays an important role in the development of alcohol liver disease. The present study was carried to evaluate the effect of grape leaf extract (GLEt) on antioxidant and lipid peroxidation states in liver and kidney alcohol induced toxicity. In vitro studies with DPPH* and ABTS*(+) (cation radical) showed that GLEt possesses antioxidant activity. In vivo administration of ethanol (7.9 g/kg bw/day) for 45 days resulted an activity of liver marker enzymes (AST, ALT, ALP and GGT), lipid peroxidation markers (TBARS, lipid hydroperoxides) in liver and kidney with significantly lower activity of SOD, CAT, GPx, GST and non-enzymatic antioxidants (vitamin E, vitamin C and GSH) in liver and kidney as compared with control rats. Administration of ethanol along with GLEt significantly decreased the activities of liver markers enzyme in serum towards near normal level. GLEt at a dose of 100 mg/kg was highly effective than 25 and 50 mg/kg body weight. In addition GLEt also significantly reduced the levels of lipid peroxidation and addition, significantly restored the enzymic and non-enzymatic antioxidants level in liver and kidney of alcohol administration rats. This observation was supplemented by histopathological examination in liver and kidney. Our data suggest that GLEt exerts its protective effect by decreased the lipid peroxidation and improving antioxidants status, thus proving itself as an effective antioxidant in alcohol induced oxidative damage in rats.

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Benzothiazoles / chemistry
  • Central Nervous System Depressants / antagonists & inhibitors*
  • Central Nervous System Depressants / toxicity*
  • Ethanol / antagonists & inhibitors*
  • Ethanol / toxicity*
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / pharmacology
  • Hydrogen Peroxide / blood
  • Kidney / pathology
  • Lipid Peroxidation / drug effects
  • Liver / pathology
  • Liver Function Tests
  • Male
  • Oxidative Stress / drug effects*
  • Phenols / analysis
  • Plant Extracts / pharmacology
  • Plant Leaves / chemistry
  • Rats
  • Rats, Wistar
  • Sulfonic Acids / chemistry
  • Thiobarbituric Acid Reactive Substances / analysis
  • Thiobarbituric Acid Reactive Substances / metabolism
  • Vitis / chemistry*

Substances

  • Antioxidants
  • Benzothiazoles
  • Central Nervous System Depressants
  • Free Radical Scavengers
  • Phenols
  • Plant Extracts
  • Sulfonic Acids
  • Thiobarbituric Acid Reactive Substances
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • Ethanol
  • Hydrogen Peroxide