Breast tumor microenvironment: proteomics highlights the treatments targeting secretome

J Proteome Res. 2008 Apr;7(4):1379-87. doi: 10.1021/pr700745n. Epub 2008 Feb 22.


Tumor secreted substances (secretome), including extracellular matrix (ECM) components, act as mediators of tumor-host communication in the breast tumor microenvironment. Proteomic analysis has emphasized the value of the secretome as a source of prospective markers and drug targets for the treatment of breast cancers. Utilizing bioinformatics, our recent studies revealed global changes in protein expression after the activation of ECM-mediated signaling in breast cancer cells. A newly designed technique integrating a capillary ultrafiltration (CUF) probe with mass spectrometry was demonstrated to dynamically sample and identify in vivo and pure secretome from the tumor microenvironment. Such in vivo profiling of breast cancer secretomes may facilitate the development of novel drugs specifically targeting secretome.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Apoptosis / drug effects
  • Breast Neoplasms / drug therapy
  • Breast Neoplasms / metabolism*
  • Breast Neoplasms / pathology
  • Extracellular Matrix / chemistry
  • Extracellular Matrix / metabolism
  • Extracellular Space / metabolism*
  • Female
  • Humans
  • Integrins / antagonists & inhibitors
  • Models, Biological
  • Proteins / analysis
  • Proteins / metabolism*
  • Proteomics / methods*


  • Integrins
  • Proteins