Diverse antimicrobial killing by Enterococcus faecium E 50-52 bacteriocin

J Agric Food Chem. 2008 Mar 26;56(6):1942-8. doi: 10.1021/jf073284g. Epub 2008 Feb 23.


An effective bacteriocin was identified and characterized. Lactic acid bacteria were screened against Campylobacter jejuni. One bacteriocin producer, Enterococcus faecium (NRRL B-30746), was studied. The isolate was grown, and the bacteriocin was purified to single-band homogeneity. Biochemical traits indicated that the peptide was a Class IIa bacteriocin, and it was named E 50-52. The bacteriocin had a molecular weight of 3339.7 and an isoelectric point of 8.0. The minimal inhibitory concentrations of E 50-52 against C. jejuni, Yersinia spp., Salmonella spp., Escherichia coli O157:H7, Shigella dysenteriae, Morganella morganii, Staphylococcus spp., and Listeria spp. ranged from 0.025 to 32 microg/mL. In therapeutic broiler trials, oral treatment with E 50-52 reduced both C. jejuni and Salmonella enteritidis by more than 100,000-fold in the ceca, and systemic S. enteritidis was reduced in the liver and spleen. The wide range of antibacterial activity of bacteriocin E 50-52 against pathogens provides a promising alternative to antibiotics.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anti-Infective Agents / chemistry
  • Anti-Infective Agents / isolation & purification
  • Anti-Infective Agents / pharmacology*
  • Bacteriocins / chemistry
  • Bacteriocins / isolation & purification
  • Bacteriocins / pharmacology*
  • Campylobacter / drug effects
  • Chickens / microbiology
  • Enterococcus faecium / growth & development
  • Enterococcus faecium / metabolism*
  • Isoelectric Point
  • Listeria / drug effects
  • Molecular Sequence Data
  • Molecular Weight
  • Sequence Alignment
  • Staphylococcus / drug effects
  • Yersinia / drug effects


  • Anti-Infective Agents
  • Bacteriocins
  • bacteriocin E 50-52, Enterococcus faecium