Oligomerization of the potato virus X 25-kD movement protein

Biochemistry (Mosc). 2008 Jan;73(1):50-5. doi: 10.1134/s0006297908010070.


A 25-kD movement protein (25K protein) encoded by the first gene of the potexvirus Potato virus X triple gene block of transport genes is essential for the viral movement in infected plants. The 25K protein belongs to superfamily 1 of NTPase/helicases and exhibits in vitro RNA helicase, Mg2+-dependent NTPase, and RNA-binding activities. In the present work, the ability of 25K protein for homologous interactions was studied using the yeast two-hybrid system, protein chemical cross-linking in the presence of glutaraldehyde, far-Western blotting, and ultracentrifugation in sucrose density gradients. The 25K protein was shown to form homodimers and homooligomers. Sites of homologous protein-protein interactions were found in both the N- and C-terminal portions of the protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dimerization
  • Glutaral / chemistry
  • Plant Viral Movement Proteins / chemistry*
  • Plant Viral Movement Proteins / metabolism
  • Potexvirus*
  • Two-Hybrid System Techniques
  • Ultracentrifugation


  • Plant Viral Movement Proteins
  • Glutaral