Characterization of an Immediate-Early Gene Induced in Adherent Monocytes That Encodes I Kappa B-like Activity

Cell. 1991 Jun 28;65(7):1281-9. doi: 10.1016/0092-8674(91)90022-q.

Abstract

We have cloned a group of cDNAs representing mRNAs that are rapidly induced following adherence of human monocytes. One of the induced transcripts (MAD-3) encodes a protein of 317 amino acids with one domain containing five tandem repeats of the cdc10/ankyrin motif, which is 60% similar (46% identical) to the ankyrin repeat region of the precursor of NF-kappa B/KBF1 p50. The C-terminus has a putative protein kinase C phosphorylation site. In vitro translated MAD-3 protein was found to specifically inhibit the DNA-binding activity of the p50/p65 NF-kappa B complex but not that of the p50/p50 KBF1 factor or of other DNA-binding proteins. The MAD-3 cDNA encodes an I kappa B-like protein that is likely to be involved in regulation of transcriptional responses to NF-kappa B, including adhesion-dependent pathways of monocyte activation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Ankyrins
  • Base Sequence
  • Blood Proteins / physiology
  • Blotting, Northern
  • Cell Adhesion
  • Cloning, Molecular
  • DNA / genetics
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / physiology
  • Gene Expression
  • Humans
  • I-kappa B Proteins*
  • Macromolecular Substances
  • Membrane Proteins / physiology
  • Molecular Sequence Data
  • Monocytes / cytology
  • Monocytes / physiology*
  • NF-KappaB Inhibitor alpha
  • NF-kappa B / antagonists & inhibitors*
  • NF-kappa B / metabolism
  • RNA, Messenger / genetics

Substances

  • Ankyrins
  • Blood Proteins
  • DNA-Binding Proteins
  • I-kappa B Proteins
  • Macromolecular Substances
  • Membrane Proteins
  • NF-kappa B
  • NFKBIA protein, human
  • RNA, Messenger
  • NF-KappaB Inhibitor alpha
  • DNA

Associated data

  • GENBANK/M69043