Engineered lentivector targeting of dendritic cells for in vivo immunization

Nat Biotechnol. 2008 Mar;26(3):326-34. doi: 10.1038/nbt1390. Epub 2008 Feb 24.


We report a method of inducing antigen production in dendritic cells by in vivo targeting with lentiviral vectors that specifically bind to the dendritic cell-surface protein DC-SIGN. To target dendritic cells, we enveloped the lentivector with a viral glycoprotein from Sindbis virus engineered to be DC-SIGN-specific. In vitro, this lentivector specifically transduced dendritic cells and induced dendritic cell maturation. A high frequency (up to 12%) of ovalbumin (OVA)-specific CD8(+) T cells and a significant antibody response were observed 2 weeks after injection of a targeted lentiviral vector encoding an OVA transgene into naive mice. This approach also protected against the growth of OVA-expressing E.G7 tumors and induced regression of established tumors. Thus, lentiviral vectors targeting dendritic cells provide a simple method of producing effective immunity and may provide an alternative route for immunization with protein antigens.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / immunology
  • Cancer Vaccines / immunology*
  • Cell Adhesion Molecules / metabolism
  • Cell Line
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism*
  • Female
  • Gene Expression
  • Genetic Vectors / genetics*
  • Humans
  • Lectins, C-Type / metabolism
  • Lentivirus / genetics*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Neoplasms, Experimental / immunology
  • Neoplasms, Experimental / therapy
  • Ovalbumin / genetics
  • Ovalbumin / immunology
  • Receptors, Cell Surface / metabolism
  • Sensitivity and Specificity
  • Sindbis Virus / genetics
  • Sindbis Virus / metabolism
  • Transduction, Genetic


  • Cancer Vaccines
  • Cell Adhesion Molecules
  • DC-specific ICAM-3 grabbing nonintegrin
  • Lectins, C-Type
  • Receptors, Cell Surface
  • Ovalbumin