Abstract
In order to investigate further the mechanisms associated with growth inhibition of human breast cancer cells by progestins and nonsteroidal antiestrogens, their effect on c-myc gene expression in T-47D-5 and T-47D cells has been investigated. The c-myc mRNA levels were differentially regulated by the synthetic progestin, medroxyprogesterone acetate and the nonsteroidal antiestrogen, monohydroxytamoxifen, in both cell lines. Antiestrogen treatment caused a persistent decrease in c-myc mRNA levels while the progestin caused a more complex response. Initially c-myc mRNA levels increased approx. 2-fold, this was followed by a decrease and then partial recovery. The end result, however, of each of these treatments is decreased cell number.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Breast Neoplasms / drug therapy
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Breast Neoplasms / genetics*
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Cell Line
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Cell Transformation, Neoplastic / drug effects
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Estrogen Antagonists / pharmacology*
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Female
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Gene Expression Regulation, Neoplastic / drug effects*
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Genes, myc*
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Humans
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Medroxyprogesterone / analogs & derivatives
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Medroxyprogesterone / pharmacology
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Medroxyprogesterone Acetate
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Progestins / pharmacology*
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Proto-Oncogene Proteins c-myc / biosynthesis
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Proto-Oncogene Proteins c-myc / genetics
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RNA, Messenger / metabolism
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Tamoxifen / analogs & derivatives
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Tamoxifen / pharmacology
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Tumor Cells, Cultured
Substances
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Estrogen Antagonists
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Progestins
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Proto-Oncogene Proteins c-myc
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RNA, Messenger
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Tamoxifen
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afimoxifene
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Medroxyprogesterone Acetate
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Medroxyprogesterone