High throughput gene expression measurement with real time PCR in a microfluidic dynamic array

PLoS One. 2008 Feb 27;3(2):e1662. doi: 10.1371/journal.pone.0001662.


We describe a high throughput gene expression platform based on microfluidic dynamic arrays. This system allows 2,304 simultaneous real time PCR gene expression measurements in a single chip, while requiring less pipetting than is required to set up a 96 well plate. We show that one can measure the expression of 45 different genes in 18 tissues with replicates in a single chip. The data have excellent concordance with conventional real time PCR and the microfluidic dynamic arrays show better reproducibility than commercial DNA microarrays.

Publication types

  • Validation Study

MeSH terms

  • Gene Expression Profiling / instrumentation
  • Gene Expression Profiling / methods*
  • Microfluidics
  • Oligonucleotide Array Sequence Analysis / standards*
  • Polymerase Chain Reaction