Epitope Mapping of 2E2-D3, a Monoclonal Antibody Directed Against Human TDP-43

Neurosci Lett. 2008 Mar 28;434(2):170-4. doi: 10.1016/j.neulet.2008.01.060. Epub 2008 Feb 2.

Abstract

TDP-43 is now known to be a major component of ubiquitin-positive, tau-negative inclusions in frontotemporal lobar degeneration with ubiquitin-positive inclusions and sporadic amyotrophic lateral sclerosis. In this study, we mapped the epitope for the monoclonal anti-TDP-43 antibody 2E2-D3. Our mapping and peptide competition experiments showed that the antibody reacted with human TDP-43, but not mouse or rat TDP-43, and recognized amino acids 205-222 of human TDP-43, corresponding to a part of the second RNA recognition motif. These findings suggest that 2E2-D3 is a useful antibody for the characterization of mouse lines transgenic for human TDP-43.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amyotrophic Lateral Sclerosis / immunology*
  • Animals
  • Antibodies, Monoclonal / genetics*
  • Antibodies, Monoclonal / immunology*
  • DNA-Binding Proteins / immunology*
  • Dementia / immunology*
  • Epitope Mapping
  • Humans
  • Mice
  • Mice, Transgenic
  • Mutagenesis
  • Rats
  • Species Specificity

Substances

  • Antibodies, Monoclonal
  • DNA-Binding Proteins