Delivery of interferon-beta to the monkey nervous system following intranasal administration

Neuroscience. 2008 Mar 27;152(3):785-97. doi: 10.1016/j.neuroscience.2008.01.013. Epub 2008 Jan 16.


We determined the nervous system targeting of interferon-beta1b (IFN-beta1b), a 20 kDa protein used to treat the relapsing-remitting form of multiple sclerosis, following intranasal administration in anesthetized, adult cynomolgus monkeys. Five animals received an intranasal bolus of [(125)I]-labeled IFN-beta1b, applied bilaterally to the upper nasal passages. Serial blood samples were collected for 45 min, after which the animals were euthanized by transcardial perfusion-fixation. High resolution phosphor imaging of tissue sections and gamma counting of microdissected tissue were used to obtain the distribution and concentration profiles of [(125)I]-IFN-beta1b in central and peripheral tissues. Intranasal administration resulted in rapid, widespread targeting of nervous tissue. The olfactory bulbs and trigeminal nerve exhibited [(125)I]-IFN-beta1b levels significantly greater than in peripheral organs and at least one order of magnitude higher than any other nervous tissue area sampled. The basal ganglia exhibited highest [(125)I]-IFN-beta1b levels among CNS regions other than the olfactory bulbs. Preferential IFN-beta1b distribution to the primate basal ganglia is a new finding of possible clinical importance. Our study suggests both IFN-beta and IFN-alpha, which share the same receptor, may be bound with relatively high affinity in these structures, possibly offering new insight into a neurovegetative syndrome induced by IFN-alpha therapy and suspected to involve altered dopamine neurotransmission in the basal ganglia. Most importantly, our results suggest intranasally applied macromolecules may bypass the blood-brain barrier and rapidly enter the primate CNS along olfactory- and trigeminal-associated extracellular pathways, as shown previously in the rat. This is the first study to finely detail the central distribution of a labeled protein after intranasal administration in non-human primates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intranasal
  • Animals
  • Autoradiography
  • Basal Ganglia / drug effects
  • Basal Ganglia / metabolism
  • Blood-Brain Barrier / drug effects
  • Blood-Brain Barrier / metabolism
  • Brain Mapping
  • Extracellular Fluid / drug effects
  • Extracellular Fluid / metabolism
  • Extracellular Space / drug effects
  • Extracellular Space / metabolism
  • Immunologic Factors / administration & dosage
  • Immunologic Factors / metabolism
  • Immunologic Factors / pharmacokinetics
  • Interferon-beta / administration & dosage
  • Interferon-beta / metabolism
  • Interferon-beta / pharmacokinetics*
  • Iodine Radioisotopes
  • Macaca
  • Male
  • Nervous System / drug effects*
  • Nervous System / immunology
  • Nervous System / metabolism
  • Olfactory Bulb / drug effects
  • Olfactory Bulb / metabolism
  • Olfactory Mucosa / drug effects*
  • Olfactory Mucosa / metabolism
  • Protein Binding / drug effects
  • Protein Binding / physiology
  • Radioimmunoassay
  • Receptors, Interferon / drug effects
  • Receptors, Interferon / metabolism
  • Trigeminal Nerve / drug effects
  • Trigeminal Nerve / metabolism


  • Immunologic Factors
  • Iodine Radioisotopes
  • Receptors, Interferon
  • Interferon-beta