A novel NAC transcription factor, IDEF2, that recognizes the iron deficiency-responsive element 2 regulates the genes involved in iron homeostasis in plants
- PMID: 18308732
- DOI: 10.1074/jbc.M708732200
A novel NAC transcription factor, IDEF2, that recognizes the iron deficiency-responsive element 2 regulates the genes involved in iron homeostasis in plants
Abstract
Iron is essential for most living organisms, and thus iron deficiency poses a major abiotic stress in crop production. Plants induce iron utilization systems under conditions of low iron availability, but the molecular mechanisms of gene regulation under iron deficiency remain largely unknown. We identified a novel transcription factor of rice and barley, IDEF2, which specifically binds to the iron deficiency-responsive cis-acting element 2 (IDE2) by yeast one-hybrid screening. IDEF2 belongs to an uncharacterized branch of the NAC transcription factor family and exhibits novel properties of sequence recognition. An electrophoretic mobility shift assay and cyclic amplification and selection of targets experiment revealed that IDEF2 predominantly recognized CA(A/C)G(T/C)(T/C/A)(T/C/A) within IDE2 as the core-binding site. IDEF2 transcripts are constitutively present in rice roots and leaves. Repression of the function of IDEF2 by the RNA interference (RNAi) technique and chimeric repressor gene-silencing technology (CRES-T) caused aberrant iron homeostasis in rice. Several genes up-regulated by iron deficiency, including the Fe(II)-nicotianamine transporter gene OsYSL2, were less induced by iron deficiency in the RNAi rice of IDEF2, suggesting that IDEF2 is involved in the regulation of these genes. Many genes with repressed expression in IDEF2 RNAi rice possessed the IDEF2-binding core sites in their promoters, and the flanking sequences were also highly homologous to IDE2. IDEF2 bound to OsYSL2 promoter region containing the binding core site, suggesting direct regulation of OsYSL2 expression. These results reveal novel cis-element/trans-factor interactions functionally associated with iron homeostasis.
Similar articles
-
The spatial expression and regulation of transcription factors IDEF1 and IDEF2.Ann Bot. 2010 Jun;105(7):1109-17. doi: 10.1093/aob/mcq002. Epub 2010 Mar 2. Ann Bot. 2010. PMID: 20197292 Free PMC article.
-
The rice transcription factor IDEF1 is essential for the early response to iron deficiency, and induces vegetative expression of late embryogenesis abundant genes.Plant J. 2009 Dec;60(6):948-61. doi: 10.1111/j.1365-313X.2009.04015.x. Plant J. 2009. PMID: 19737364
-
Identification of novel cis-acting elements, IDE1 and IDE2, of the barley IDS2 gene promoter conferring iron-deficiency-inducible, root-specific expression in heterogeneous tobacco plants.Plant J. 2003 Dec;36(6):780-93. doi: 10.1046/j.1365-313x.2003.01920.x. Plant J. 2003. PMID: 14675444
-
The Molecular Mechanisms Underlying Iron Deficiency Responses in Rice.Int J Mol Sci. 2019 Dec 19;21(1):43. doi: 10.3390/ijms21010043. Int J Mol Sci. 2019. PMID: 31861687 Free PMC article. Review.
-
The Adaptive Mechanism of Plants to Iron Deficiency via Iron Uptake, Transport, and Homeostasis.Int J Mol Sci. 2019 May 16;20(10):2424. doi: 10.3390/ijms20102424. Int J Mol Sci. 2019. PMID: 31100819 Free PMC article. Review.
Cited by
-
Genome-wide association study and transcriptome analysis discover new genes for bacterial leaf blight resistance in rice (Oryza sativa L.).BMC Plant Biol. 2021 Jun 3;21(1):255. doi: 10.1186/s12870-021-03041-2. BMC Plant Biol. 2021. PMID: 34082694 Free PMC article.
-
Defects in the rice aconitase-encoding OsACO1 gene alter iron homeostasis.Plant Mol Biol. 2020 Dec;104(6):629-645. doi: 10.1007/s11103-020-01065-0. Epub 2020 Sep 9. Plant Mol Biol. 2020. PMID: 32909184
-
Discovery of a conserved translationally repressive upstream open reading frame within the iron-deficiency response regulator IDEF2.BMC Plant Biol. 2024 Sep 30;24(1):891. doi: 10.1186/s12870-024-05473-y. BMC Plant Biol. 2024. PMID: 39343926 Free PMC article.
-
Validation of a high-confidence regulatory network for gene-to-NUE phenotype in field-grown rice.Front Plant Sci. 2022 Nov 25;13:1006044. doi: 10.3389/fpls.2022.1006044. eCollection 2022. Front Plant Sci. 2022. PMID: 36507422 Free PMC article.
-
Rice genes involved in phytosiderophore biosynthesis are synchronously regulated during the early stages of iron deficiency in roots.Rice (N Y). 2013 Jun 25;6(1):16. doi: 10.1186/1939-8433-6-16. Rice (N Y). 2013. PMID: 24280375 Free PMC article.
Publication types
MeSH terms
Substances
Associated data
- Actions
- Actions
LinkOut - more resources
Full Text Sources
Medical
