Effect of propolis on healing in experimental colon anastomosis in rats

Adv Ther. 2008 Feb;25(2):159-67. doi: 10.1007/s12325-008-0013-2.


Introduction: Propolis is the generic name for the resinous substance collected by honeybees, which is known to have antioxidant, anti-inflammatory, apoptosis-inducible effects. Anastomotic dehiscence after colorectal surgery is an important cause of morbidity and mortality. We aimed to assess the effect of propolis on healing in an experimental colon anastomosis in rats.

Methods: Forty adult male Wistar albino rats were randomly assigned into 5 treatment groups with 8 rats in each: Group I, anastomosis+no treatment; Group II, anastomosis+oral propolis (600 mg/kg/d); Group III, anastomosis+oral ethyl alcohol (1 cc/d); Group IV, anastomosis+rectal propolis (600 mg/kg/d); Group V, anastomosis+rectal ethyl alcohol (1 cc/d). The bursting pressures, hydroxiproline levels and histopathological changes in each group were measured.

Results: When bursting pressures were compared between groups, we observed that they were increased in the groups treated with propolis in contrast to all other groups. Hydroxiproline levels in the propolis groups were also significantly increased in contrast to the other groups. There was also a statistically significant difference in histopathological changes between the treatment types. When propolis administration methods were compared, we did not observe a statistically significant difference.

Conclusion: Propolis has a significantly favourable effect on healing in experimental colon anastomosis, independent from the method of administration.

MeSH terms

  • Anastomosis, Surgical
  • Animals
  • Colon / surgery*
  • Male
  • Propolis / pharmacology*
  • Rats
  • Rats, Wistar
  • Surgical Wound Dehiscence / physiopathology
  • Wound Healing / drug effects*


  • Propolis