Chlamydia-like bacteria in respiratory samples of community-acquired pneumonia patients

FEMS Microbiol Lett. 2008 Apr;281(2):198-202. doi: 10.1111/j.1574-6968.2008.01099.x. Epub 2008 Feb 27.


Chlamydia-like bacteria, obligate intracellular relatives of Chlamydia trachomatis and Chlamydophila pneumoniae, are widely distributed in nature. Using a two-step nested and semi-nested PCR approach targeting the 16S rRNA gene, we found DNA of Chlamydia-like bacteria in respiratory samples from patients with community-acquired pneumonia. Of 387 cases tested, four (1.03%) tested positive if only sequences showing less than 99.9% 16S rRNA gene sequence similarity to known Chlamydiae were considered. These included for the first time Protochlamydia amoebophila, Waddlia chondrophila, and 'Candidatus Rhabdochlamydia porcellionis'-related sequences. This study extends previous findings suggesting an association of Chlamydia-like bacteria with respiratory disease, but a causal link between these microorganisms and respiratory tract infections has yet to be established.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Chlamydia / genetics
  • Chlamydia / isolation & purification*
  • Chlamydia Infections / microbiology*
  • Community-Acquired Infections / microbiology*
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Female
  • Humans
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Pneumonia / microbiology*
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sequence Homology


  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/EU090706
  • GENBANK/EU090707
  • GENBANK/EU090708
  • GENBANK/EU090709