A sensitive and specific liquid chromatography-mass spectrometry method for determination of metacavir in rat plasma

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Mar 15;864(1-2):9-14. doi: 10.1016/j.jchromb.2007.12.022. Epub 2008 Jan 4.

Abstract

A sensitive and specific liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) method has been developed and validated for the quantification of metacavir in rat plasma using tinidazole as an internal standard (I.S.). Following ethyl acetate extraction, the analytes were separated on a Shim-pack ODS (4.6 microm, 150 mm x 2.0 mm I.D.) column and analyzed in selected ion monitoring (SIM) mode with a positive ESI interface using the respective [M+H](+) ions, 266 for metacavir and 248 for tinidazole. The method was validated over the concentration range of 1-600 ng/mL for metacavir. Between and within-batch precisions (R.S.D.%) were all within 15% and accuracy (%) ranged from 92.2 to 105.8%. The lower limit of quantification (LLOQ) was 1 ng/mL. The extraction recovery was on average 89.8%. The validated method was used for the pharmacokinetic study of metacavir in rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antiviral Agents / blood*
  • Antiviral Agents / pharmacokinetics
  • Chromatography, Liquid / methods*
  • Drug Stability
  • Purine Nucleosides / blood*
  • Purine Nucleosides / pharmacokinetics
  • Rats
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrometry, Mass, Electrospray Ionization / methods*

Substances

  • 2-amino-9-(2,3-dideoxy-2,3-dihydroarabinofuranosyl)-6-methoxy-9H-purine
  • Antiviral Agents
  • Purine Nucleosides