Dethiobacter alkaliphilus gen. nov. sp. nov., and Desulfurivibrio alkaliphilus gen. nov. sp. nov.: two novel representatives of reductive sulfur cycle from soda lakes

Extremophiles. 2008 May;12(3):431-9. doi: 10.1007/s00792-008-0148-8. Epub 2008 Mar 4.

Abstract

Anaerobic enrichments with H2 as electron donor and thiosulfate/polysulfide as electron acceptor at pH 10 and 0.6 M total Na+ yielded two non sulfate-reducing representatives of reductive sulfur cycle from soda lake sediments. Strain AHT 1 was isolated with thiosulfate as the electron acceptor from north-eastern Mongolian soda lakes and strain AHT 2-with polysulfide as the electron acceptor from Wadi al Natrun lakes in Egypt. Both isolates represented new phylogenetic lineages: AHT 1-within Clostridiales and AHT 2-within the Deltaproteobacteria. Both bacteria are obligate anaerobes with respiratory metabolism. Both grew chemolithoautotrophically with H2 as the electron donor and can use thiosulfate, elemental sulfur and polysulfide as the electron acceptors. AHT 2 also used nitrate as acceptor, reducing it to ammonia. During thiosulfate reduction, AHT 1 excreted sulfite. dsrAB gene was not found in either strain. Both strains were moderate salt-tolerant (grow up to 2 M total Na+) true alkaliphiles (grow between pH 8.5 and 10.3). On the basis of the phenotypic and phylogenetic data, strains AHT 1 and AHT 2 are proposed as new genera and species Dethiobacter alkaliphilus and Desulfurivibrio alkaliphilus, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological
  • Ammonia / metabolism
  • Bacteria / classification
  • Bacteria / genetics
  • Bacteria / growth & development
  • Bacteria / isolation & purification
  • Bacteria / metabolism*
  • Chemoautotrophic Growth
  • Cytochromes / metabolism
  • DNA, Bacterial / analysis*
  • Deltaproteobacteria / classification
  • Deltaproteobacteria / genetics
  • Deltaproteobacteria / growth & development
  • Deltaproteobacteria / isolation & purification
  • Deltaproteobacteria / metabolism*
  • Egypt
  • Fatty Acids / metabolism
  • Fresh Water / chemistry
  • Fresh Water / microbiology*
  • Hydrogen-Ion Concentration
  • Kenya
  • Molecular Sequence Data
  • Nitrates / metabolism
  • Oxidation-Reduction
  • Phenotype
  • Phylogeny
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Salinity
  • Sulfides / metabolism*
  • Thiosulfates / metabolism*
  • Time Factors

Substances

  • Cytochromes
  • DNA, Bacterial
  • Fatty Acids
  • Nitrates
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Sulfides
  • Thiosulfates
  • Ammonia
  • polysulfide

Associated data

  • GENBANK/EF422412
  • GENBANK/EF422413