An interleukin-1 receptor antagonist protein protects insulin-producing beta cells against suppressive effects of interleukin-1 beta

Diabetologia. 1991 Jun;34(6):445-8. doi: 10.1007/BF00403185.


The cytokine interleukin-1 beta may have an important role in the autoimmune mediated damage of pancreatic Beta cells in insulin-dependent diabetes mellitus. In the present study we have investigated the effects of an interleukin-1 receptor antagonist protein, a blocker of the type I interleukin-1 receptor, on the suppressive actions of recombinant interleukin-1 beta on insulin-producing cells. Brief exposure (1-2 h) of rat and mouse pancreatic islets to 10 ng/ml recombinant interleukin-1 beta induced an 70-80% inhibition of insulin response to glucose after 12 h. These effects were completely counteracted by co-incubation with 100 ng/ml interleukin-1 receptor antagonist protein. When rat islets were cultured for 48 h in the presence of recombinant interleukin-1 beta (5 ng/ml) higher concentrations of interleukin-1 receptor antagonist protein (5000 ng/ml) were required to protect Beta-cell function. Interleukin-1 receptor antagonist protein also counteracted the inhibitory effects of recombinant interleukin-1 beta on the growth of the rat insulinoma cell line RINm5F. These data suggest that interleukin-1 receptor antagonist protein can protect insulin-producing cells from the deleterious effects of recombinant interleukin-1 beta, and that these cells possess type I interleukin-1 receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Disease Models, Animal
  • Glucose / physiology
  • Insulin / metabolism*
  • Insulinoma / metabolism
  • Insulinoma / pathology
  • Insulinoma / ultrastructure
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1 / pharmacology*
  • Islets of Langerhans / cytology*
  • Islets of Langerhans / metabolism
  • Islets of Langerhans / ultrastructure
  • Male
  • Mice
  • Pancreatic Neoplasms / metabolism
  • Pancreatic Neoplasms / pathology
  • Pancreatic Neoplasms / ultrastructure
  • Proteins / pharmacology*
  • Rats
  • Rats, Inbred Strains
  • Receptors, Immunologic / drug effects
  • Receptors, Immunologic / physiology
  • Receptors, Interleukin-1
  • Recombinant Proteins / pharmacology
  • Sialoglycoproteins*
  • Time Factors
  • Tumor Cells, Cultured / drug effects
  • Tumor Cells, Cultured / pathology
  • Tumor Cells, Cultured / ultrastructure


  • Il1rn protein, mouse
  • Insulin
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1
  • Proteins
  • Receptors, Immunologic
  • Receptors, Interleukin-1
  • Recombinant Proteins
  • Sialoglycoproteins
  • Glucose