Efficient immobilization and patterning of live bacterial cells

Langmuir. 2008 Apr 15;24(8):4161-7. doi: 10.1021/la7038653. Epub 2008 Mar 6.


A monolayer of live bacterial cells has been patterned onto substrates through the interaction between CFA/I fimbriae and the corresponding antibody. Patterns of live bacteria have been prepared with cellular resolution on silicon and gold substrates for Salmonella enterica serovar Typhimurium as a model with high specificity and efficiency. The immobilized cells are capable of dividing in growth medium to form a self-sustaining bacterial monolayer on the patterned areas. Interestingly, the immobilized cells can alter their orientation on the substrate, from lying-down to standing-up, as a response to the cell density increase during incubation. This method was successfully used to sort a targeted bacterial species from a mixed culture within 2 h.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cells, Immobilized
  • Escherichia coli / chemistry*
  • Escherichia coli / cytology*
  • Microbial Viability
  • Microscopy, Atomic Force
  • Salmonella typhimurium / chemistry*
  • Salmonella typhimurium / cytology*