Inability of real-time reverse transcriptase PCR assay to detect subtype H7 avian influenza viruses isolated from wild birds

J Clin Microbiol. 2008 May;46(5):1844-6. doi: 10.1128/JCM.02426-07. Epub 2008 Mar 5.

Abstract

We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed for detecting H7 influenza viruses in poultry, is not reliable for wild bird surveillance.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Birds
  • DNA Primers / genetics
  • False Negative Reactions*
  • Influenza A virus / genetics
  • Influenza A virus / isolation & purification*
  • Influenza in Birds / diagnosis*
  • Influenza in Birds / virology*
  • Mutation
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity

Substances

  • DNA Primers
  • RNA, Viral