Overexpression of a TFIIIA-type zinc finger protein gene ZFP252 enhances drought and salt tolerance in rice (Oryza sativa L.)

FEBS Lett. 2008 Apr 2;582(7):1037-43. doi: 10.1016/j.febslet.2008.02.052. Epub 2008 Mar 4.

Abstract

We previously identified a salt and drought stress-responsive TFIIIA-type zinc finger protein gene ZFP252 from rice. Here we report the functional analysis of ZFP252 using gain- and loss-of-function strategies. We found that overexpression of ZFP252 in rice increased the amount of free proline and soluble sugars, elevated the expression of stress defense genes and enhanced rice tolerance to salt and drought stresses, as compared with ZFP252 antisense and non-transgenic plants. Our findings suggest that ZFP252 plays an important role in rice response to salt and drought stresses and is useful in engineering crop plants with enhanced tolerance to salt and drought stresses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Metabolism
  • Dehydration
  • Gene Expression
  • Genes, Plant
  • Oryza / genetics*
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plants, Genetically Modified / drug effects
  • Plants, Genetically Modified / metabolism
  • Proline / metabolism
  • Sodium Chloride / pharmacology
  • Transcription Factor TFIIIA / genetics
  • Transcription Factor TFIIIA / metabolism*

Substances

  • Plant Proteins
  • Transcription Factor TFIIIA
  • Sodium Chloride
  • Proline