Functional alteration of macrophages by a slime-forming Lactococcus lactis ssp. cremoris

J Dairy Sci. 1991 Jul;74(7):2082-8. doi: 10.3168/jds.S0022-0302(91)78380-X.


The effect of a slime-forming, encapsulated Lactococcus lactis ssp. cremoris KVS20 on macrophage function has been examined in vivo and in vitro in short-term studies. Peritoneal macrophages in which 21 to 34% of macrophage was presenting Fc gamma-receptor positive macrophages were elicited by intraperitoneal injection of 10 to 50 mg/kg of L. lactis ssp. cremoris KVS20. The peritoneal macrophage exhibited cytotoxic activity against Sarcoma-180 cells in which the maximum activity was obtained in macrophage from mice injected with 10 mg/kg on d 5. However, L. lactis ssp. cremoris KVS20 rendered the elicited macrophage cytotoxic in vitro. The cytotoxicity was significantly augmented by 6- and 24-h treatment at the concentration of 50 to 500 micrograms/ml. These results obtained in the short-term studies demonstrated that the antitumor activity of L. lactis ssp. cremoris KVS20 may be mediated through the enhanced cytotoxic activity of macrophage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Cytotoxicity, Immunologic*
  • Lactococcus lactis / physiology*
  • Macrophages / immunology
  • Macrophages / microbiology*
  • Male
  • Mice
  • Mice, Inbred ICR
  • Peritoneal Cavity / cytology
  • Peritoneal Cavity / microbiology
  • Phagocytosis*
  • Sarcoma 180
  • Specific Pathogen-Free Organisms
  • Tumor Cells, Cultured