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Guideline
. 2008 Mar;26(3):305-12.
doi: 10.1038/nbt1391.

Minimum Information Specification for in Situ Hybridization and Immunohistochemistry Experiments (MISFISHIE)

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Free PMC article
Guideline

Minimum Information Specification for in Situ Hybridization and Immunohistochemistry Experiments (MISFISHIE)

Eric W Deutsch et al. Nat Biotechnol. .
Free PMC article

Abstract

One purpose of the biomedical literature is to report results in sufficient detail that the methods of data collection and analysis can be independently replicated and verified. Here we present reporting guidelines for gene expression localization experiments: the minimum information specification for in situ hybridization and immunohistochemistry experiments (MISFISHIE). MISFISHIE is modeled after the Minimum Information About a Microarray Experiment (MIAME) specification for microarray experiments. Both guidelines define what information should be reported without dictating a format for encoding that information. MISFISHIE describes six types of information to be provided for each experiment: experimental design, biomaterials and treatments, reporters, staining, imaging data and image characterizations. This specification has benefited the consortium within which it was developed and is expected to benefit the wider research community. We welcome feedback from the scientific community to help improve our proposal.

Figures

Figure 1
Figure 1
The six sections of the MISFISHIE specification.
Figure 2
Figure 2
An abbreviated checklist for the full MISFISHIE specification. This checklist should be used in conjunction with the full specification, not instead of it.

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