Reduction of oxidative stress marker levels by anti-TNF-alpha antibody, infliximab, in patients with rheumatoid arthritis

Clin Exp Rheumatol. Jan-Feb 2008;26(1):73-80.

Abstract

Objective: The aim of this study was to evaluate the effects of anti-TNF-alpha antibody, infliximab, on oxidative stress markers representing DNA damage, lipid peroxidation, and glycoxidation.

Methods: Twenty-three RA patients underwent infliximab treatment and were analyzed for 30 weeks. Six patients who experienced side effects and one patient who had a reduced efficacy of infliximab were discontinued the infliximab treatment at 30-54 weeks. Sixteen patients were analyzed for 54 weeks. The levels of serum total, urinary total, and free pentosidine, which is an advanced glycation end-product (AGE), and of urinary 15-Isoprostane F2t and 8-hydroxy-deoxy guanosine (8-OHdG) were determined at baseline and at 14, 30, and 54 weeks after initial treatment with infliximab.

Results: Serum total, urinary total, and free pentosidine levels were reduced at 54 weeks after initial infliximab treatment. Urinary 15-Isoprostane F2t and 8-OHdG levels were also reduced at 14, 30, and 54 weeks. Urinary 8-OHdG levels in RA patients correlated with CRP and the Disease Activity Score of 28 joints.

Conclusion: In RA patients, infliximab plays an essential role as an anti-oxidative agent against AGE formation, oxidative DNA damage and lipid peroxydation.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Antibodies, Monoclonal / pharmacology*
  • Antibodies, Monoclonal / therapeutic use
  • Antirheumatic Agents / pharmacology*
  • Antirheumatic Agents / therapeutic use
  • Arginine / analogs & derivatives*
  • Arginine / analysis
  • Arginine / blood
  • Arginine / urine
  • Arthritis, Rheumatoid / drug therapy*
  • Arthritis, Rheumatoid / metabolism
  • DNA Damage / drug effects
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / urine
  • Female
  • Glycation End Products, Advanced / metabolism
  • Humans
  • Infliximab
  • Isoprostanes / urine*
  • Lipid Peroxidation
  • Lysine / analogs & derivatives*
  • Lysine / analysis
  • Lysine / blood
  • Lysine / urine
  • Male
  • Middle Aged
  • Oxidative Stress
  • Reactive Oxygen Species

Substances

  • Antibodies, Monoclonal
  • Antirheumatic Agents
  • Glycation End Products, Advanced
  • Isoprostanes
  • Reactive Oxygen Species
  • 8-Hydroxy-2'-Deoxyguanosine
  • Arginine
  • Infliximab
  • pentosidine
  • Deoxyguanosine
  • Lysine