Simultaneous determination of two Amadori compounds in Korean red ginseng (Panax ginseng) extracts and rat plasma by high-performance anion-exchange chromatography with pulsed amperometric detection

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Apr 1;865(1-2):159-66. doi: 10.1016/j.jchromb.2008.02.012. Epub 2008 Feb 23.


A new simple, rapid and sensitive high-performance anion-exchange chromatography method with pulsed amperometric detection (HPAEC-PAD) was developed and validated for the simultaneous determination of two Amadori compounds, arginyl-fructose and arginyl-fructosyl-glucose in Korean red ginseng (Panax ginseng) extracts, rat plasma. Separation of the two target analytes was efficiently undertaken on CarboPac PA1 anion-exchange column with isocratic elution (400 mM sodium hydroxide and deionized water (90:10, v/v)) at flow rate 0.7 mL/min within 15 min of single chromatographic run. Under optimized conditions, the detection limits (signal-to-noise ratio equal to 3) were 20 and 25 ng/mL for arginyl-fructose and arginyl-fructosyl-glucose, respectively. Calibration curves of peak area for the two analytes were linear over three orders of magnitude with a correlation coefficients greater than 0.999. The accuracy of the method was tested by recovery measurement of the spiked samples which yielded good results of 94.15-102.62%. This method was successfully applied to the quantification of arginyl-fructose and arginyl-fructosyl-glucose in herbal extracts and in the plasma samples from rat.

Publication types

  • Validation Study

MeSH terms

  • Animals
  • Anion Exchange Resins
  • Calibration
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, Ion Exchange / methods*
  • Electrochemistry / methods*
  • Panax / chemistry*
  • Plant Proteins / isolation & purification
  • Rats
  • Reproducibility of Results
  • Sensitivity and Specificity


  • Anion Exchange Resins
  • Plant Proteins