Expression cloning of the human IL-3 receptor cDNA reveals a shared beta subunit for the human IL-3 and GM-CSF receptors

Cell. 1991 Sep 20;66(6):1165-74. doi: 10.1016/0092-8674(91)90039-2.


A cDNA for a human interleukin-3 (hIL-3) binding protein has been isolated by a novel expression cloning strategy: a cDNA library was coexpressed with the cDNA for the beta subunit of human granulocyte/macrophage colony-stimulating factor (GM-CSF) receptor (hGMR beta) in COS7 cells and screened by binding of 125I-labeled IL-3. The cloned cDNA (DUK-1) encodes a mature protein of 70 kd, which belongs to the cytokine receptor family and which alone binds hIL-3 with extremely low affinity (Kd = 120 +/- 60 nM). A high affinity IL-3-binding site (Kd = 140 +/- 30 pM) was reconstituted by coexpressing the DUK-1 protein and hGMR beta, indicating that hIL-3R and hGMR share the beta subunit. Therefore, we designated DUK-1 as the alpha subunit of the hIL-3R. As in human hematopoietic cells, hIL-3 and hGM-CSF complete for binding in fibroblasts expressing the cDNAs for hIL-3R alpha, GMR alpha, and the common beta subunit, indicating that different alpha subunits compete for a common beta subunit.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding, Competitive
  • Blotting, Northern
  • Cloning, Molecular
  • Cross-Linking Reagents
  • DNA / genetics
  • Gene Expression
  • Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
  • Humans
  • Interleukin-3 / metabolism
  • Interleukins / metabolism
  • Macromolecular Substances
  • Molecular Sequence Data
  • Molecular Structure
  • RNA, Messenger / genetics
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / chemistry
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor / genetics*
  • Receptors, Interleukin-3 / chemistry
  • Receptors, Interleukin-3 / genetics*
  • Recombinant Proteins


  • Cross-Linking Reagents
  • Interleukin-3
  • Interleukins
  • Macromolecular Substances
  • RNA, Messenger
  • Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
  • Receptors, Interleukin-3
  • Recombinant Proteins
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • DNA

Associated data

  • GENBANK/M74782