Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast

Cell. 1991 Sep 20;66(6):1197-206. doi: 10.1016/0092-8674(91)90042-w.


We have isolated a number of cDNAs derived from human mRNAs that are able to substitute for G1 cyclin genes in S. cerevisiae. Several of these encode human cyclins A, B1, and B2. Three novel genes have been identified, which we call cyclins C, D, and E. The novel proteins are sufficiently distantly related to the other members of the cyclin family and to each other as to constitute three new classes of cyclins. Cyclin C and E mRNAs accumulate periodically through the cell cycle, peaking at different times in G1.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cell Cycle*
  • Cloning, Molecular
  • Cyclins / genetics*
  • DNA / genetics
  • Gene Expression
  • Genetic Complementation Test
  • HeLa Cells
  • Humans
  • Mitosis
  • Molecular Sequence Data
  • Oligonucleotides / chemistry
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • Saccharomyces cerevisiae / genetics
  • Species Specificity


  • Cyclins
  • Oligonucleotides
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/M74091
  • GENBANK/M74092
  • GENBANK/M74093