Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family

Cell. 1991 Sep 20;66(6):1217-28. doi: 10.1016/0092-8674(91)90044-y.


A new human cyclin, named cyclin E, was isolated by complementation of a triple cln deletion in S. cerevisiae. Cyclin E showed genetic interactions with the CDC28 gene, suggesting that it functioned at START by interacting with the CDC28 protein. Two human genes were identified that could interact with cyclin E to perform START in yeast containing a cdc28 mutation. One was CDC2-HS, and the second was the human homolog of Xenopus CDK2. Cyclin E produced in E. coli bound and activated the CDC2 protein in extracts from human G1 cells, and antibodies against cyclin E immunoprecipitated a histone H1 kinase from HeLa cells. The interactions between cyclin E and CDC2, or CDK2, may be important at the G1 to S transition in human cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • CDC2 Protein Kinase / physiology*
  • Cell Cycle*
  • Cyclins / classification
  • Cyclins / physiology*
  • DNA / genetics
  • Enzyme Activation
  • Genetic Complementation Test
  • Humans
  • Molecular Sequence Data
  • RNA, Messenger
  • Saccharomyces cerevisiae / genetics
  • Sequence Alignment


  • Cyclins
  • RNA, Messenger
  • DNA
  • CDC2 Protein Kinase

Associated data

  • GENBANK/M73812