We evaluated 4 suggested treatments for cyanoacrylate (CA) eyelash adhesion. Nylon paintbrushes were used to simulate human eyelashes. Brushes were treated with one drop of CA and allowed to dry. The brushes were randomly assigned to one of 6 treatment groups: Bacitracin, water, K-Y Jelly, baby shampoo, acetone (+control), and no treatment (-control). Two blinded investigators independently rated each brush to determine if the treatment decreased the adhesion of the bristles. Six brushes were assessed for each therapy. No treatment option was superior to control, and all treatments except acetone had no effect on bristle adherence. Our results suggest these treatments are unlikely to be clinically useful for removing CA from eyelashes.